Extended Data Fig. 3: Adrenal medulla potency and pseudotime.
From: Single-cell transcriptomic analyses provide insights into the developmental origins of neuroblastoma
a, Diffusion map of adrenal medullary cells as shown in Fig. 2a, colored by 19 clusters of transcriptionally similar cells. Start of the pseudotime trajectory is marked with an arrow (cluster 16). b, c, UMAP embedding of adrenal medullary cells without cycling populations colored by 19 clusters of transcriptionally similar cells (b) or cell types (c). Start of the pseudotime trajectory is marked with an arrow (cluster 16). d, f, UMAP plot showing adrenal medullary cells without cycling populations colored by differentiation potential as determined by CytoTRACE (d) and differentiation potency as determined by LandSCENT (f). e, Boxplot with differentiation potencies for adrenal medullary cell populations as determined by LandSCENT. Here, the center line represents the median, upper and lower boundaries the 75th and 25th percentile and whiskers the 1.5x interquartile range. Number of cells: late SCPs: n = 632, SCPs: n = 817, cycling SCPs: n = 288, Bridge: n = 612, con. Prog: n = 1499, Chrom: n = 1346, late Chrom: n = 963, cycling Nbs: n = 1064, Nbs: n = 1388, late Nbs: n = 2130. g, Diffusion map of adrenal medullary cells colored by SCP-to-late SCP pseudotime trajectory as determined by slingshot. h, UMAP visualization illustrating adrenal medullary cells without cycling populations colored by pseudotime as determined by monocle3. Start of the pseudotime trajectory is marked with an arrow. i, Gene expression of selected genes over pseudotime in chromaffin cell and neuroblast trajectories.
