Extended Data Fig. 9: Mutagenesis of the endogenous hs122 enhancer results in dentate gyrus abnormalities (a smaller dentate gyrus with disorganized appearance).
From: Ultraconserved enhancer function does not require perfect sequence conservation
Rostral (a) and caudal (b) coronal cross-sections of postnatal hippocampus from all phenotyped animals: three wild-type littermate male controls and four hemizygous knock-in males shown side-by-side for comparison. Numbers represent unique identifiers for each mouse examined (details in Supplementary Table 6). Scale bars, 500 μm. c, Bar graph showing quantification of dentate gyrus length for wild-type mice (n = 3) and knock-in mice (n = 4). Bars indicate mean lengths, with individual biological replicates represented by dots. Error bars, mean ± s.d. Difference in length between wild-type and knock-in mice assessed by a two-tailed paired t-test.
