Extended Data Fig. 4: Comparison of the effects of Zrsr2 loss versus Tet2 knockout or Sf3b1K700E or Srsrf2P95H mutations on hematopoietic stem and progenitor cells.
From: Minor intron retention drives clonal hematopoietic disorders and diverse cancer predisposition
a, Schema of competitive bone marrow (BM) transplantation assays. b, Absolute number of CD45.2+ long-term HSCs (LT-HSCs), LSK, and MPPs in the bone marrow of CD45.1 recipient mice 16 weeks following pIpC (n = 8–10 each). For box and whiskers plots throughout, bar indicates median, box edges first and third quartile values, and whisker edges minimum and maximum values. c, Percentage of CD45.2+ LT-HSCs, LSK, CMP, MEP, and GMP cells in the BM of CD45.1 recipient mice 16-weeks following pIpC (n = 8–10 per each). P value was calculated relative to the control group by a two-sided t-test. d, Representative FACS plots of data in (c). e, Number of methylcellulose colonies generated from 100 sorted LT-HSCs from mice with the indicated genotype. n = 3 biologically independent experiments. Error bars, mean values +/− SEM. P-values by one-way ANOVA with Tukey’s multiple comparisons test. f, Percentage of CD45.2+ cells in the blood of recipient mice from Zrsr2 knockout/Sf3b1K700E/WT double mutant cells and relevant controls pre- and post-pIpC administration to recipient mice (n = 10 each). P-values by two-way ANOVA with Tukey’s multiple comparisons test. Data in (b), (c), and (f) are shown as box-and-whisker plots where bar indicates median, box edges first and third quartile values, and whisker edges minimum and maximum values.
