Extended Data Fig. 6: Zfp410 is the conserved mouse ortholog of ZNF410.

a, CUT&RUN performed in mouse erythroleukemia (MEL) cells using anti-Zfp410 antibody (n = 3) and IgG control (n = 3). The third most enriched Zfp410 peak (following Chd4 promoter and Chd4 -6 kb enhancer) was at the Hist1h2bl promoter. No Zfp410 motifs were identified at this locus, which overlaps accessible chromatin (DNase-seq, gray peaks). b, Diagram of the Zfp410 gene trap allele. A targeting cassette including splice acceptor site upstream of LacZ was inserted into Zfp410 intron 5 thus disrupting full-length expression. Schema obtained along with mouse ES cells from EuMMCR, Germany. c, Exon and domain structure of mouse Zfp410. d, Mouse embryonic (βh1 and εy) and adult β-major/minor globin gene expression measured by RT-qPCR in Zfp410 Gt/Gt (n = 5) mouse E14.5 fetal liver erythroid cells compared to heterozygous (n = 4) and wildtype (n = 5) control animals. e, Weight was measured at indicated time points over the course of 15 weeks for wildtype male (+/+ (M), n = 1), Zfp410 heterozygous male (+/Gt (M), n = 2), Zfp410 homozygous male (Gt/Gt (M), n = 2), Zfp410 heterozygous female (+/Gt (F), n = 5) and Zfp410 homozygous female (Gt/Gt (F), n = 1) mice. Data are presented as mean values and error bars are standard deviation. f, Peripheral blood hematological parameters for wildtype (n = 1), Zfp410 + /Gt (n = 7) and Zfp410 Gt/Gt (n = 3) mice, with normal ranges for hemoglobin, mean corpuscular volume (MCV), reticulocyte, white blood cell (WBC), neutrophil and platelet count shown by dotted lines.