Extended Data Fig. 7: ZNF410 is dispensable for hematopoietic repopulation and erythropoiesis.
From: ZNF410 represses fetal globin by singular control of CHD4
CD34 + HSPCs from donor 3 were edited by RNP electroporation targeting ZNF410, BCL11A or ZBTB7A and infused to NBSGW mice or subject to in vitro erythroid differentiation. a, Indel frequency at ZNF410, BCL11A and ZBTB7A was quantified in input cells 4 days after electroporation, and in engrafted total or sorted cells at bone marrow (BM) harvest. The percentage of frameshift alleles is represented in gray and the percentage of in-frame alleles is represented in white. b, Comparison of engraftment assessed by human CD45 + staining compared to total CD45 + cells in xenografts of ZNF410 (n = 4), BCL11A (n = 3) and ZBTB7A (n = 3) edited and mock control (n = 4) CD34 + HSPCs. Each symbol represents one mouse. c,d, Erythroid maturation, evaluated based on CD71 and CD235a immunophenotype and enucleation frequency, was assessed on day 18 of in vitro erythroid culture in safe control (n = 4), ZNF410 (n = 2), BCL11A (n = 2) and ZBTB7A (n = 2) targeted primary erythroblasts.
