Extended Data Fig. 4: Empirical distribution of ABE editing efficiency and DNA sequence motifs measured by ATAC-seq.
From: Single-nucleotide-level mapping of DNA regulatory elements that control fetal hemoglobin expression
a, Empirical distribution of ABEmax editing activities in HUDEP-2 cells. Bar plot shows the average editing activity at different positions among 23 different ABEmax edited loci. X-axis denotes positions relative to protospacer start (position 1). Y-axis shows the A to G conversion rate. b, A heatmap of on-target base-editing efficiencies of ABEmax as measured by targeted amplicon sequencing of 23 different edited genomic loci (row). Each cell represents one nucleotide. The cell number indicates the relative position of the nucleotide relative to the PAM sequence. The editing efficiency was measured by determining the percentage of nucleotide converted by ABEmax. c, The footprint profiles of GATA1, ZBTB7A, and CTCF binding sites derived from deep sequencing (ATAC-seq). The heatmap represents the ATAC-seq signals within a ±100-bp window for the top 1000 binding sites for each TF. Each row represents one binding site. Aggregated signals are plotted in the top panels.
