Extended Data Fig. 6: 3D Live-Seq dataset of a PDTO-9 organoid consisting of 23 cells. | Nature Genetics

Extended Data Fig. 6: 3D Live-Seq dataset of a PDTO-9 organoid consisting of 23 cells.

From: Reconstructing single-cell karyotype alterations in colorectal cancer identifies punctuated and gradual diversification patterns

Extended Data Fig. 6

a, 3D Live-Seq dataset of a PDTO-9 organoid consisting of 23 cells of which 21 cells were whole-genome sequenced (91% recovery). Top: representative stills of the growing PDTO-9 structure with nuclei in false color depth code. Below: full mitotic tree with 3D-rendered stills of each anaphase. Onset of anaphase is indicated by arrowheads in relation to the time axis (in hours). Bottom panel shows a karyotype heatmap of all cells of the imaged PDTO. Lineage I: one cell carries four copies of Chr1q24.2-ter and three cells carry the reciprocal loss. b, Minimal phylogenic structure of lineage I, which only fits branch 1.2 (highlighted in orange) of the mitotic tree. Obtaining four copies of Chr1q24.2-ter involves one round of replication and collective missegregation of the Chr1q fragments (Fig. 3).

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