Extended Data Fig. 7: Enhancer dynamics in H3.3-K27M NSC cultures.

a. Ranked Order of Super Enhancer (ROSE) analysis in WT and K27M-mutant NSC cultures for embryo GCGR-NS13. b. Bar charts presenting the relative shift in the number of typical and super enhancer elements in WT and K27M-mutant hindbrain NSC cultures, n = 2 biologically independent samples. c. Tornado plots of averaged ChIP-Rx normalised H3K27ac signal ±10kb of the centre of active promoters and enhancer elements in duplicate WT and K27M-mutant NSC cultures. d. Meta-tracks of ChIP-Rx normalised signal for the indicated antibodies at active promoter and enhancer regions in duplicate WT and K27M-mutant NSC cultures. e. Waterfall plots presenting the log₂ fold-change in H3K27ac ChIP-Rx signal at all identified active enhancers (top) and active promoters (bottom) between WT and K27M-mutant NSC cultures. f. Box plots presenting the log₂ fold-change in H3K27ac ChIP-Rx signal at active promoters and enhancers separated into their respective top (Q5), and bottom (Q1) quintiles based on H3.3-K27M abundance, n = 2 biologically independent samples. Box plots present median values and interquartile ranges (IQR), with whiskers representing quartiles 1/3 ± (1.5 × IQR) respectively. g. Genomic tracks showing quantitative H3K27ac ChIP-Rx signal around the HOXA gene cluster on chromosome 7 (chr7:26,811,403-27,600,402). h. Meta-tracks of ChIP-seq normalised H3K27M signal at active promoter and enhancer regions in two independent K27M mutant patient derived DMG cell lines. One sample is H3.1-K27M, while the second is H3.3-K27M.