Extended Data Fig. 1: An isogenic hindbrain neural stem cell DMG model. | Nature Genetics

Extended Data Fig. 1: An isogenic hindbrain neural stem cell DMG model.

From: Simultaneous disruption of PRC2 and enhancer function underlies histone H3.3-K27M oncogenic activity in human hindbrain neural stem cells

Extended Data Fig. 1

a. Immunoblots in cell lysates from unmanipulated NSCs and lines expressing WT or K27M-mutant H3.3. Representative example of n = 2 data. b. Absolute quantitative PCR of endogenous/exogenous H3F3A transcripts in NSCs expressing WT or K27M H3F3A, n = 3 independent samples. c. Immunoblots in cell lysates from WT or K27M H3.3 NSC cultures. Representative example of n = 2 data. d. Immunoblots (Left) and quantifications (Right) of total H3 levels in NSCs expressing WT or K27M H3F3A, n = 4 independent samples. Presented as mean values ± SD. e. Immunoblots in NSCs expressing WT or K27M H3F3A or H3K27M mutant DIPG cell lines. Representative example of n = 2 data. f. Relative quantitative PCR analysis of INK4A levels in NSCs expressing WT or K27M H3.3, n = 2 independent samples. g. Growth analysis of H3.3 WT and K27M expressing NSCs, n = 3 independent samples. Presented as mean values ± SD.

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