Extended Data Fig. 2: Tumorigenic potential of hindbrain NSC DMG model. | Nature Genetics

Extended Data Fig. 2: Tumorigenic potential of hindbrain NSC DMG model.

From: Simultaneous disruption of PRC2 and enhancer function underlies histone H3.3-K27M oncogenic activity in human hindbrain neural stem cells

Extended Data Fig. 2

a. EdU positivity in PP5W and PP5K cultures derived from embryos GCGR-NS19 and GCGR-NS13, n = 4 independent samples. Presented as mean values ± SD. b. Colony forming activity in PP5W and PP5K cultures, n = 6 independent samples. Data are median values and interquartile ranges (IQR), with whiskers representing quartiles 1/3 ± (1.5 × IQR) respectively. c. Senescence Associated ß-Galactosidase (SA-ß-Gal) positivity in PP5W and PP5K cultures, n = 3 independent samples. Presented as mean values ± SD. d. Mouse survival following stereotactic injection of PP5W or PP5K NSCs, n = 4 PP5W and n = 8 PP5K mice. e. Brightfield and GFP micrographs of wholemount/cross-sectional views of a representative mouse brain injected with PP5K cells. f. Immunohistochemistry of brain samples from mice transplanted with PP5W and PP5K cells. Transplanted V5-tag (H3.3) positive cells display a diffuse pattern in mouse tissue, with no defined margins between human cells and the mouse brain parenchyma. Scale bar: 1.5mm. Representative example of n = 4 data. g. Immunohistochemistry of V5-tag (H3.3) and Ki67 in brain tissue from mice transplanted with PP5W and PP5K cells. Bar plot shows percentage of proliferating transplanted cells (KI67±V5-tag+) in each group. Scale bar: 20mM, n = 4 WT and n = 3 K27M biological samples. Presented as mean values ± SD.

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