Extended Data Fig. 8: The structural variation of CCR gene (GhM_A02G1731 versus Ghir_A02G014590).

a, The location of structural variation in the genome of NDM8 against TM-1. b, The structural variation led to the difference in the open reading frame (ORF) between NDM8 and TM-1, and the conservative structure domain (NAD_binding_10) of CCR in NDM8. c, Three-dimensional structure of CCR (GhM_A02G1731) was obtained by homologous modeling. The second deletion (508–552) in TM-1 influenced the formation of CCR structure within NAD-binding domain that was indicated by red dotted line. d, Expression of CCR in resistant (NDM8) and susceptible (TM-1) cotton varieties under V. dahliae stress through qRT–PCR. Ghhistone3b was used as an internal control. e, Comparison of CCR genomic sequences among ancestral diploid species and cultivated tetraploid cottons. f, Comparison of CCR partial coding sequences among ancestral diploid species and cultivated tetraploid cottons.