Extended Data Fig. 2: Developmental mutation rates averages to 2-4 SNVs across entire genome per cell doubling.
From: Synonymous mutations reveal genome-wide levels of positive selection in healthy tissues
(a) SNV VAFs in HSPC single-cell colonies in an 8 - week foetus30 where coverage is 22.6x per colony. SNVs found between 35% - 65% (within the dashed lines) are likely clonal in the colony. (b) SNV VAFs in HSPC single-cell colonies in an 18 - week foetus30 where coverage is 12.2x per colony. SNVs found between 30% - 70% (within the dashed lines) are likely clonal in the colony. (c) The best-fit to the reverse cumulative for the number of mutations per cell doubling per haploid is 1.86 (95% CI = 1.6 - 2.1) for Lee Six et al. data (green line and datapoints), 1.0 (95% CI = 1.0-1.1) for Chapman et al. 8-week foetus (purple line and datapoints) and 1.0 (95% CI = 0.9-1.1) for Chapman et al. 18-week foetus (orange line and datapoints).
