Extended Data Fig. 7: Hoxb cluster genes exhibit interdependent switching of transcriptional states in response to transient activation.
From: A cis-acting mechanism mediates transcriptional memory at Polycomb target genes in mammals
a, Messenger RNA levels of indicated target genes in cells bearing the DD-dCas9-VPR construct and either lacking (wild-type, Ezh2 KO) or constitutively expressing (VPR OFF, ON, washout) sgRNAs specific to the target gene. Values within each experiment are normalized to the Ezh2 KO condition. Horizontal lines represent mean values. b, Left, RNA-seq and nascent-RNA-seq over the Hoxb cluster (chromosome 11: 96,182,001-96,373,000) for the indicated conditions in iMEF B, with the position of the long-Hoxb transcript shown below. Right, as in a, but for long-Hoxb. c, Messenger RNA levels of indicated target genes in cells bearing the DD-dCas9-VPR construct and either lacking (wild-type, Ezh2 KO) or constitutively expressing (VPR OFF, ON, washout) sgRNAs specific to long-Hoxb.
