Extended Data Fig. 7: Spatially-resolved single-cell transcriptomic expression of WNT and NOTCH signals.

a, High-resolution large-area imaging of uterine tissue sections stained with smFISH for WNT7A, WNT5A, and LGR5. White arrowheads indicate magnified regions demonstrating spatial segregation of WNT7A (lumenal epithelial) and WNT5A (stromal) expression. Scale bars, top = 200 μm, other = 50 μm. b, High-resolution imaging of endometrial tissue sections stained with smFISH for JAG1. A comparison of representative regions of lumenal and glandular epithelium is shown for three secretory phase donors. Scale bars = 10 μm. c, Co-staining of JAG1 and HEY1. Top, solid white arrowheads indicate regions of lumenal epithelium magnified below. Below, cells showing juxtacrine expression of JAG1 and HEY1 (magenta arrowheads = JAG1^highHEY1^low, green arrowheads = JAG1^lowHEY1^high) or co-expression (white outlined arrowheads). Scale bars, top = 50 μm, other = 5 μm. d, Dot plot showing log₂-transformed expression of AXIN2 expression.