Extended Data Fig. 9: NOTCH and WNT inhibition.
From: Mapping the temporal and spatial dynamics of the human endometrium in vivo and in vitro
a, Representative brightfield images of the organoids treated with DBZ, DAPT, IWP-2 or XAV939 at the end of the experiment (day 6). Scale bars, 500 μm, 200 μm, 100 μm from left to right. Black arrowheads point at folded organoids. Representative images of three endometrial organoids from three different donors. b, UMAP (uniform manifold approximation and projection) projections of scRNA-seq data from all organoid samples. c, UMAP projections of scRNA-seq data coloured by days after hormonal stimulation, individual, hormonal stimulation, inhibitor used, phase of the cell cycle and sample ID. d, quantitative real-time PCR (qRT-PCR) analysis for genes expressed in ciliated or secretory cells of hormonally-stimulated organoids treated with NOTCH inhibitors (blue) or WNT inhibitors (pink). Bar plots showing the mean with SD levels of expression relative to housekeeping genes and control conditions at day 0 (without inhibitors, black) (n = 3 different donors). Deviations to the control conditions (without inhibitors) were detected as statistically significant by Dunnett’s multiple comparisons with p-value <0.05, which defines two-sided confidence intervals for individual deviations. e, ELISA assay for glycodelin (PAEP) from supernatants of hormonally stimulated organoids treated with NOTCH (blue) or WNT inhibitors (pink). Bar plots showing the mean with SD levels of expression (n = 3 donors). Deviations to the control condition (no inhibitors, no hormones) at day 0 were detected as statistically significant with Dunnett’s multiple comparisons (p-value <0.05).
