Fig. 4: Cell signaling in glandular and lumenal epithelium.
From: Mapping the temporal and spatial dynamics of the human endometrium in vivo and in vitro
a, Heatmaps showing TFs differentially expressed in ciliated (top) and secretory (bottom) epithelial lineages. Color is proportional to log-transformed fold change; asterisks highlight TFs whose targets are also differentially expressed (that is, differentially activated TFs). b, Unbiased clustering of epithelial subsets using Visium data. Spot colors represent cluster assignment based on Louvain clustering of spots assigned to epithelial subsets. Spots assigned to one of the clusters (represented in light gray in the figure) were excluded from the analysis due to the low percentage of epithelial cells in the spot after visual inspection. c, Heatmap showing log-transformed fold change of differentially expressed genes between the three main clusters defining the lumenal, functional and basal epithelial regions. d, High-resolution large-area imaging of a representative section of secretory-phase endometrium, with pseudocolor intensity proportional to smFISH signal for NOTCH2. Representative image of three endometrial samples from three different donors. e, smFISH quantification in three full-thickness secretory-phase endometrial samples. Plots represent smFISH spot intensity in glands divided by gland area at increasing distances from the lumen. Approximate lumen range is marked in yellow.
