Extended Data Fig. 10: Correction by genome editing of mutated CCL22 reverts the higher expression of chemokine/cytokine and their receptors in primary CLPD-NK samples. | Nature Genetics

Extended Data Fig. 10: Correction by genome editing of mutated CCL22 reverts the higher expression of chemokine/cytokine and their receptors in primary CLPD-NK samples.

From: CCL22 mutations drive natural killer cell lymphoproliferative disease by deregulating microenvironmental crosstalk

Extended Data Fig. 10

NK cell line NKL which has the hotspot CCL22 mutation (Arg44_Leu45insSer) was edited (CRISPR/Cas9) to revert the mutation (mt) into the wild type (wt) codon. a, Relative gene expression of chemokine receptors in CCL22 mutant compared to wild type was shown. CCL22 mutant NKL represented higher expression of several chemokine receptors. b, Comparison of chemokine and cytokine secretion between CCL22 mutant (NKL-parent-CCL22mt) and wild type NKL (NKL-edited-CCL22wt) by using chemokine array. Cell culture media after 72-hour incubation was analyzed. Several cytokines showed enhanced secretion in CCL22 mutant that related to dendritic cell maturation.

Back to article page