Extended Data Fig. 3: Downstream mapping of genes and chromatin sites controlled by each IL2RA regulator.

a, mRNA fold change for the CRISPR targeted gene in each knockout sample. Data are presented as the effect size from Limma, with error bars showing the 95% confidence interval. b, Comparison of average changes in IL2RA mRNA levels (RNA-Seq) and protein levels (flow cytometry) for each knockout sample collected for RNA-Seq and ATAC-Seq. c, Percent of significantly changed ATAC-Seq peaks in each knockout sample that contain a known motif for the knocked out transcription factor. d,e, The total number of significantly changed genes (d) or peaks (e) detected via RNA-Seq and ATAC-Seq in each knockout sample. For a-e, n = 3 donors for the RNA-Seq and ATAC-Seq data. f, Summary of changes in IL2RA levels measured using flow cytometry. For each knockout, the change in IL2RA median fluorescence intensity is normalized to AAVS1 knockout alone controls. g, The percent of reads containing insertions/deletions (indels) at the genomic target sites for the guide RNAs and samples in f. Solid line indicates the mean indel percentage across different perturbation combinations.