Extended Data Fig. 10: High-resolution MS revealed the protein changes in Xbp1-/- embryos.
a, Heatmap illustrating the proteins that were differentially expressed in Xbp1−/− embryos and wild-type embryos (the colors represent the z-scores of the protein expression). A two-sided t-test is performed for comparing protein levels of KO embryos to WT embryos (p-value ≤ 0.05, fold change ≥ 1.5). b, Volcano plot showing the differentially expressed proteins in Xbp1−/− embryos and WT embryos. The lines mark thresholds for log values of the p-value and fold change. The dots of text annotations are genes that are canonical Xbp1 targets related to the unfolded protein response (UPR). The yellow and blue dots are genes with significantly upregulated genes in KO embryos and WT embryos respectively. c, mESCs and Xbp1−/− mESCs grown in mESCs medium for 3 days and showing no visible differences in cell morphology. The experiment was replicated three times with similar results. Scale bar, 50 μm. d, qPCR analysis of Nanog, Oct4, and Sox2 expression in mESCs and Xbp1−/− mESCs showing no significant differences (normalized by the expression level of Gapdh, n = 3 per box). A two-sided Wilcoxon rank sum test is performed for comparing gene expression levels of wild-type and knockout mESCs (p-value ≥ 0.05: not significant, mean ± s.d.).
