Extended Data Fig. 5: Validation of additional SARS-CoV-2 restriction factors enriched in the GOF screen. | Nature Genetics

Extended Data Fig. 5: Validation of additional SARS-CoV-2 restriction factors enriched in the GOF screen.

From: Genome-wide bidirectional CRISPR screens identify mucins as host factors modulating SARS-CoV-2 infection

Extended Data Fig. 5

a. RT-qPCR analysis of target-gene expression for indicated GOF cell lines graphed as expression fold change over NTG for each gene. Statistical significance is measured by individual two-sided t-tests comparing GOF lines to NTG, n = 3 biological replicates. Dotted line indicates NTG expression level (normalized to 1). b-d. Viral titer in GOF cell lines infected with SARS-CoV-2 at an MOI of 0.05 for 48 hours and measured by TCID50 assay. Dotted line indicates the limit of detection (LOD) of the assay, as well as the NTG average. Statistical significance is measured by individual two-sided t-tests comparing GOF lines to NTG, n = 3 biological replicates. For all panels: error bars denote mean ± s.e.m., significance is indicated as: n.s.=not significant, *= P < 0.05, **= P < 0.01, ***=P < 0.001, ****= P < 0.0001.

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