Extended Data Fig. 10: Membrane-tethered and gel-forming mucins modulate infection of multiple respiratory viruses. | Nature Genetics

Extended Data Fig. 10: Membrane-tethered and gel-forming mucins modulate infection of multiple respiratory viruses.

From: Genome-wide bidirectional CRISPR screens identify mucins as host factors modulating SARS-CoV-2 infection

Extended Data Fig. 10

a. Incucyte trace tracking GFP+ (PIV3 infected) cells over 72 hours in Calu-3 mucin GOF cell lines, either pretreated with StcE or not, n = 5 biological replicates. Full dataset for the 48-hour time point from Fig. 8h. b-c. Plaque assay measuring viral loads of the indicated Calu-3 GOF cells infected with HKU5-SARS-Cov-1, or MERS-CoV, respectively, at 24 hpi at an MOI of 0.1. Dotted line indicates the NTG average for guides tested. Significance was calculated by individual two-sided t-tests comparing each cell line to NTG, n = 3 biological replicates. d-e. qRT-PCR measuring viral gene copies 24 hpi in mucin-overexpressing cell lines for 229E (MOI 0.05), or PIV3 (MOI 0.0002), respectively. Dotted line indicates the NTG average. Significance was calculated by individual two-sided t-tests comparing each cell line to NTG, n = 3 biological replicates. f. GFP+ (infected) cells from indicated GOF Calu-3 cell lines infected with PIV3 at MOI 0.0002 at 40 hpi. Significance was calculated by individual two-sided t-tests comparing each cell line to NTG, n = 3 biological replicates. Dotted line indicates the NTG average.g. Incucyte trace tracking GFP + infected cells from (f). For all panels: error bars denote mean ± s.e.m., significance is indicated as: n.s.=not significant, *= P < 0.05, **= P < 0.01, ***=P < 0.001, ****= P < 0.0001.

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