Fig. 2: Random segregation of ecDNA promotes intratumoral heterogeneity of oncogenes in cancer cell lines and patient tumor samples.
From: The evolutionary dynamics of extrachromosomal DNA in human cancers
a, Schematic showing the quantification of ecDNA copy number heterogeneity from simulations of random ecDNA segregation and ecDNA+ cell lines. b, ecDNA oncogene copy number measured by interphase FISH in cancer cell lines. Agreement between observed (colored histograms) and simulated (dashed histograms) revealed that oncogene copy number heterogeneity largely follows the predicted distribution. Unadjusted P values from Shapiro–Wilks and Kolmogorov–Smirnov tests are shown. c, Schematic showing the quantification of ecDNA copy number heterogeneity from simulations of random ecDNA segregation and ecDNA+ patient data. d, ecDNA copy number distribution in six patients with GBM (dots) e, and four patients with NB (dots) emerges from the same process of random ecDNA segregation (black dashed line).
