Extended Data Fig. 1: Prom1+ P1KP-GAC cells propagate gastric adenocarcinoma as allografts.
From: The NALCN channel regulates metastasis and nonmalignant cell dissemination
(a) Growth of allografts derived from three independent P1KP gastric adenocarcinomas (GAC) in immunocompromised mice (PROM1+ n = 4 and PROM1−n = 3 for each allograft). Statistics are based on Permutation test whereby permuted p-values compare PROM1+ and PROM1− allografts for each tumour type using the average t-statistic between the groups as the test statistic. Two-tailed Permutation test GAC1 p = 0.044, GAC2 p = 0.05, GAC3 p = 0.028. (b) Histology of primary P1KP-GAC and daughter PROM1+ cell allografts. Tumours expressed gastric adenocarcinoma-associated lectins and PROM1 identified by Beta-galactosidase staining from Prom1 CreERT2/LacZ locus (scale=100um). Representative photomicrographs Primary P1KP-GAC n = 3 and PROM1+ cell allografts n = 12 evaluated histologically.
