Extended Data Fig. 2: Recombination of the NalcnFlx conditional allele deletes the gene in vivo.
From: The NALCN channel regulates metastasis and nonmalignant cell dissemination
(a) Polymerase chain reaction (PCR) products derived from brains of Nalcn+/+, Nalcn+/Flx, or NalcnFlx/Flx mice with or without the NestinCre allele. (b) Real-time reverse transcriptase PCR analysis of Nalcn RNA expression in brains of Nalcn+/+ (n = 4), Nalcn+/Flx (n = 6), or NalcnFlx/Flx (n = 4) mice carrying the NestinCre allele. bar=median (*p = 0.0190 Nalcn+/Flx, *p = 0.0286 NalcnFlx/Flx; two-tailed Mann-Whitney U Test). (c) Similar to germline deletion of Nalcn, homozygous deletion of Nalcn from the brains of NestinCre;NalcnFlx/Flx (n = 6) mice was lethal at birth due to respiratory arrest, providing functional validation of Nalcn deletion. control (n = 20) and NestinCre;Nalcn+/Flx (n = 11).
