Fig. 2: Increased channel open probability due to destabilization of the X-gate.
From: Gain-of-function mutations in KCNK3 cause a developmental disorder with sleep apnea
a, Representative single-channel recordings of DDSA mutants at a holding potential of −160 mV. b, Single-channel Po values for each mutant recorded at −160 mV. WT (n = 3), N133S (n = 3), L239P (n = 4), G129D (n = 4), L122V (n = 4), L241F (n = 4) and L122P (n = 5). All mutant Po values differ from that for WT (P < 0.01, two-paired t-test). Single-channel conductance measurements for each mutant are also reported in Supplementary Table 1. c, Plot of the minimum pore radius at the lower X-gate during three independent repeats of molecular dynamics simulations of the WT TASK-1 structure compared to the N133S and L239P mutant structures. These variants destabilize the closed X-gate structure, allowing the channel to open more frequently.
