Extended Data Fig. 7: EGLN2 regulates MDA-MD-231 cell proliferation but not 293T cells.
From: Histone H3 proline 16 hydroxylation regulates mammalian gene expression
a, Display of Venn diagram showing overlapping of genes being affected between the MDA-MB-231 breast cancer cells and 293 T cells. b-e, Immunoblots for lysates (b), cell proliferation assays (c), representative images of soft agar assays(d) and quantification of soft agar assays(e) in MDA-MB-231 cells transduced with lentivirus expressing either sgCtrl or EGLN2 sg13/14. f-i, Immunoblots for lysates (f), cell proliferation assays (g), representative images of soft agar assays(h) and quantification of soft agar assays(i) in 293 T cells transduced with lentivirus expressing either sgCtrl or EGLN2 sg13/14. For panel c, e, g and i, the y axis shows averaged values after normalization to sgCtrl (n = 3; mean ± s.d.; unpaired two-tailed Student’s t-test). #/*P < 0.05, ##/**P < 0.01, ###/***P < 0.001.
