Extended Data Fig. 10: EGLN2-catalyzed H3P16oh leads to decreased H3K4me3 in physiologic and physiological context.
From: Histone H3 proline 16 hydroxylation regulates mammalian gene expression
a, Presentation of box plots of EGLN2 expression between normal (N) and breast cancer tumors (T, BRCA) in TCGA data analyzed by GEPIA2. Box and whisker plots show all points, minimum to maximum, with 25th to 75th interquartile range box, the center line indicates the median, and the whiskers indicate 1.5 × the interquartile range. Normal (n = 291) versus Tumor (n = 1085). b, Dot plots of EGLN2 expression across all tumor samples(T, red) and paired normal tissues(N, green) in TCGA data analyzed by GEPIA2. For panel a-b, Log2FC Cutoff=1, p-value Cutoff=0.01, ManneWhitney U-tests. c, Immunoblots of expression level of EGLN2 and H3P16oh in indicated breast cell lines. d, Immunoblots of lysates from paired patient-derived normal (N) and tumor (T) breast tissues. e, Immunoblots of expression level of H3P16oh and indicated histone makers in different EGLN2+/+ and EGLN2−/− mice tissues. The values listed below the blots indicate the relative protein levels with H3 normalization, the protein expression level from EGLN2+/+ tissues were set as 1.0. Values in red denotes ‘upregulation’, values in green denotes ‘downregulation’ and values in bold denotes ‘no obvious change’ (EGLN2−/− vs EGLN2+/+). ND, not detected. f, Table of the summary for protein quantification. g, Immunoblots of expression level of H3P16oh and indicated histone makers in EGLN2+/+ and EGLN2−/− female mice mammary gland tissues. The values listed below the blots indicate the relative protein levels with H3 normalization. For panel e and g, we do quantitative comparisons between samples on different blots, the samples derive from the same experiment and that blots were processed in parallel. h, Dot plots of the values of relative protein expression level with H3 normalization in EGLN2+/+ and EGLN2−/− female mice breast tissues from panel g. n = 10, mean ± s.d.; unpaired two-tailed Student’s t-test; *P < 0.05, **P < 0.01, ***P < 0.001.
