Extended Data Fig. 4: Depletion of Prrx1+ cells leads to defects in homeostasis and repair of bone and skin.
From: Prrx1 marks stem cells for bone, white adipose tissue and dermis in adult mice
(a, b) The numbers of Prrx1 lineage cells were decreased by TAM and DT injection into 3-month-old Prrx1-CreERT2;iDTR;tdTomato male mice. Stromal cells of iWAT and dermis were released by protease digestion and then analysed with flow cytometry (a), while bone sections of the mice were stained with DAPI (b). Scale bar: 250 μm. Each experiment was repeated three times independently with similar results obtained. (c) Representative images showed comparison of bone (μCT), iWAT, and dermis of Rosa-iDTR mice treated with DT or solvent (Veh). Scale bar: 250 μm for the bone and 50 μm for the iWAT and dermis. Each experiment was repeated three times independently with similar results obtained. (d) H/E staining showed that the growth plate and articular cartilage appeared normal in Prrx1-CreERT2; iDTR mice after depletion of Prrx1+ cells. Scale bar: 50 μm. The experiment was repeated with 6 mice for each group. (e) Depletion of Prrx1+ stem cells led to defects in gonadal and mesenteric adipose tissues. Scale bar: 50 μm. Each experiment was repeated with 3 mice for each group with similar results obtained. (f) A time-course study of the repair of skin puncture wounds when Prrx1+ stem cells were depleted. Bottom panel: quantitation data. Data are presented as mean ± S.D. N = 6 mice. P values were calculated by two-tailed Student’s t-tests. (g) Representative images showed comparison of skin wound healing of Rosa-iDTR mice treated with DT or Veh. (h) Representative images showed comparison of bone fracture healing of Rosa-iDTR mice treated with DT or Veh.
