Fig. 1: TET3 is required to maintain 5hmC levels in SMCs.
From: Spurious transcription causing innate immune responses is prevented by 5-hydroxymethylcytosine
a, LacZ staining of cryosections derived from different organs of Tet3floxLacZ/+ mice (n = 3). Scale bars, 50 μm (heart and lung) or 20 μm (brain and muscle). b, H&E staining of paraffin sections from control or Tet3smKO lungs (n = 5). Scale bar, 200 μm (upper panel); 50 μm (middle panel); 10 μm (lower panel). The experimental design is depicted in the upper panel. c, Outline of the strategy to isolate tdTomato-labeled SMCs. d, Western blot analysis of TET3 in sorted lung control and Tet3smKO:T SMCs. e, Dot blot analysis of 5mC (n = 4), 5hmC (n = 3), 5fC and 5caC (n = 4) levels in sorted lung SMCs from control and Tet3smKO:T mice. Methylene blue (MB) staining served as loading control. f, Quantification of 5mC, 5hmC, 5fC and 5caC levels from the dot blot analysis of SMCs (two-tailed unpaired t-test). n represents independent animals. Data are presented as mean values ± s.e.m.
