Fig. 6: TET3 prevents TLR7-dependent inflammatory responses by formation of 5hmC.
From: Spurious transcription causing innate immune responses is prevented by 5-hydroxymethylcytosine
a, Dot blot analysis of 5hmC levels in mESC-SMCs after Tet3 knockdown (Tet3KD) with or without expression of human TET3WT or TET3CD (n = 3 independent experiments). Quantification of 5hmC levels is shown on the right (n = 3; one-way ANOVA with Tukey’s post hoc test: **P < 0.01, ***P < 0.001). The experimental strategy for manipulation of mESC-SMCs is depicted in the upper panel. b, RT-qPCR analysis of Tlr7, Irf7, Il1b Cd80, Cd86 and Cxcl9 in mESC-SMCs after transduction of scramble, Tet3KD, Tet3KD + TET3WT and Tet3KD + TET3CD lentiviruses (n = 4 independent experiments; one-way ANOVA with Tukey’s post hoc test: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). c, RT-qPCR analysis of HeLa cells transfected with RNA isolated from mESC-SMCs after transduction with scramble, Tet3KD, Tet3KD + TET3WT and Tet3KD + TET3CD lentiviruses with or without TLR7 inhibitor (TLR7in) E6446 (n = 3 independent experiments; one-way ANOVA with Tukey’s post hoc test: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). Data in a–c are presented as mean values ± s.e.m.
