Extended Data Fig. 5: Levels of RNAPII or epigenetic markers around the TSS (±750 bp) of each defined functional gene cluster, related to Fig. 4.
From: Genome-wide RNA polymerase stalling shapes the transcriptome during aging
Functional gene clusters: promoter-upregulated genes, promoter-downregulated genes, genes with a high gradual loss of productive transcription (GLPThigh) and remainder. Data are mean ± s.d. Blue lines represent adult liver, red lines represent old liver. Average of n = 3 / group shown for: a, Total RNAPII. b, serine 5 phosphorylated (ser5p) RNAPII. c, histone 3 lysine 27 acetylation (H3K27Ac; open chromatin). d, histone 3 lysine 4 trimethylation (H3K4Me3; open chromatin). e, inaccessible chromatin as MNase digests only DNA not bound to proteins including nucleosomes.
