Fig. 5: Transcriptional stress affects mRNA output and aging-related pathways.
From: Genome-wide RNA polymerase stalling shapes the transcriptome during aging
a,b, Sequencing density profiles of the entire Igf1 gene (mm10, chr10:87,858,265–87,937,047), including RNAPII coding strand bias (a) and exons only (b) from EU-seq of adult (blue) and aged (red) WT mouse liver. Exons 1a and 1b are alternative start sites. c, EU-seq density ratios between last and first exons for all expressed genes (P = 5.06731 × 10−9), short (10–22 kb) and long genes (>110 kb, P = 0.000482946). Data are the mean ± s.e.m. P values are from a two-sided unpaired t-test (old versus adult). d, Full transcript abundances (relative to adult) estimated by reads covering 3′UTR from EU-seq of all expressed genes (P = 0.048761825), short (10–22 kb) and long genes (>110 kb, P = 1.78654 × 10−6). Data are the mean ± s.e.m., P values are from a two-sided unpaired t-test. e, Significant overrepresented pathways in TShigh genes by IPA, KEGG, Reactome and GSEA-hallmarks classified by main process category (bold). Aggregated P values were obtained from a Fisher’s exact test. See Supplementary Table 2 for detailed pathway information.
