Fig. 5: High relative content of small circular DNAs is associated with DNA damage response pathway activation.
From: Parallel sequencing of extrachromosomal circular DNAs and transcriptomes in single cancer cells
a, Density plot of relative small circular DNA (<3 kb) content in CHP-212 single cells (n = 129). For differential expression analyses, cells were divided in two categories: ‘low’ (orange area, bottom 40%) and ‘high’ (purple area, top 40%). b, Violin plot comparing the relative number of small circular DNAs (<3 kb) at different cell cycle phases in CHP-212 (red, n = 129) and TR14 (blue, n = 20) single cells. A two-sided Welch’s t-test was used among the indicated conditions. P values are shown. c, Cellular processes significantly enriched in CHP-212 cells with high relative very small circular DNA content. Adjusted P values and gene counts are shown. d, Gene set enrichment analysis (GSEA) plot of genes involved in DNA repair (adjusted P = 0.0415). e, GSEA plot of genes involved in the cellular response to the DNA damage stimulus (adjusted P = 0.0008). P values were adjusted using the Bejamini–Hochberg method.
