Fig. 6: scEC&T-seq detects circular DNAs in primary neuroblastomas at the single-cell level.
From: Parallel sequencing of extrachromosomal circular DNAs and transcriptomes in single cancer cells
a, Schematic diagram describing tumor and blood sample processing. b, Number of individual circular DNA regions normalized by library size detected in primary tumor nuclei (n = 93 nuclei patient no. 1, n = 86 nuclei patient no. 2), neuroblastoma cell line single cells (n = 25 TR14 cells, n = 150 CHP-212 cells) and nonmalignant single T cells (n = 38 patient no. 3, n = 41 patient no. 4). P values were calculated using a two-sided Welch’s t-test and are shown. The boxes in the boxplots represent the 25th and 75th percentiles with the center bar as the median value and the whiskers representing the furthest outlier ≤1.5× the IQR from the box. c, Heatmap of the genome-wide circular DNA density in neuroblastoma primary tumors and normal T cells (n = 93 patient no. 1, green; n = 86 patient no. 2, purple; n = 38 patient no. 3, yellow; n = 41 patient no. 4, orange; bin sizes = 3 Mb). The location of the MYCN gene in chr2 is shown.
