Fig. 4: Multiple cancer-associated aneuploidy events can significantly improve growth rate.
From: Chromosome evolution screens recapitulate tissue-specific tumor aneuploidy patterns
a,b, Correlation of growth rates (PDs per day) for HMEC (a) and RPTEC (b) aneuploid clones compared to average CNA frequencies in cognate tumor types, breast cancer (a) and renal cancer (b). Parental diploid population growth rates are indicated by horizontal dotted black lines, ± standard error of the mean (green shading). a, Pearson’s correlation coefficient squared (r2 = 0.60) and associated P value (P = 0.070) are shown. Dashed line indicates linear regression model of the data. b, Pearson’s correlation coefficient squared (r2 = 0.26) and P = 0.078. c, Growth rates of evolved lineages that gained combinations of +20, +8q and/or +1q compared to pre-evolved isogenic ancestors. P values calculated from two-sided Wilcoxon tests. Solid black line indicates median diploid control growth rate. rev, reversion. d, Correlation of the growth rate differences (D, delta) between evolved and ancestor clones, relative to ancestor clone growth rate. Colors indicate time to clonal sweep of CNAs (in PDs). Plus signs indicate copies gained (one plus sign, one copy). Pearson’s correlation coefficient squared (r2 = 0.80) and associated P value (P = 0.016) are shown. Dashed gray line indicates linear regression model of the data. e, Bar graphs showing Hallmark GSEA scores (signed −log10(false discovery rate)) of differentially expressed gene sets in newly aneuploidized (pre-evolved) clones (dark blue) and in post-evolved aneuploids (light blue), each compared to diploid controls. All copy number-specific effects on gene expression were normalized before analysis. Gene sets with differential expression are grouped on the basis of their relative behavior in pre- and post-evolved aneuploid cells. EMT, epithelial-to-mesenchymal transition; Ox. phos., oxidative phosphorylation; UV, ultraviolet.
