Extended Data Fig. 5: Zic1/Zic4 and Mir9-2 brain enhancer knock-outs.
From: Increased enhancer–promoter interactions during developmental enhancer activation in mammals
a, Map of the deleted region encompassing hs654 midbrain enhancer of Zic1/Zic4 together with H3K27ac, DNase-seq, ATAC-seq from midbrain and conservation track across 60 species. b, Sanger sequencing of the PCR product from hs654 knock-out mice (n = 4 biological replicates). c, representative PCR genotyping results of the hs654 enhancer knockout mice. Lanes in the gel were rearranged so that results for wild-type and heterozygous mice are adjacent to each other. d, Map of the deleted region encompassing hs267, hs266 and hs853 forebrain enhancers of Mir9-2 together with H3K27ac, DNase-seq, ATAC-seq from midbrain and conservation track across 60 species. e, Sanger sequencing of the PCR product from hs267-853 knock-out mice (n = 3 biological replicates). f, representative PCR genotyping results of the hs267-853 enhancer knockout mice. g, Genotype frequency data for enhancer knockout lines. Mice homozygous for either deletion were born at normal Mendelian ratios, and no gross phenotypes or impairments were observed. P-values were calculated using the one-sided chi-square test.
