Extended Data Fig. 7: Data supporting activated EGF(-like) signaling activity.

a, Subset of DEGs (n = 2 STR^mut vs n = 5 NL STR^wt thyroids) that were directionally consistent with EGF signaling activity (from the analysis shown in Fig. 7e). b, Expression values of DEGs shown in a. Gene expression profile of an STR^wt (nontoxic) MNG is shown for comparison. c, Downregulation of HPN encoding an EGFR-inactivating protease. Specific suppression of HPN expression in STR^mut thyroid glands by RNA-seq (n = 2 thyroids with STR^mut and 5 NL with STR^wt) and RT-qPCR. For RT-PCR, samples included n = 4 NL thyroids (2 distinct from RNAseq samples), n = 3 with AITD, n = 2 with PTC, and one with MNG (all STR^wt). For both Pt1 and Pt2 with STR^mut, two separate specimens from their excised thyroid glands were analyzed by qPCR. ****, FDR = 2 × 10⁻²¹ (DESeq2). d, Alignment of MIR7-5P to predicted 3′-UTR target sites⁹⁴ of HPN. Watson–Crick pairings are shown as vertical dashes and G:U wobble pairings by dots. TPM, transcripts per million.

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