Extended Data Fig. 5: Domain structures and sequences of proteins involved in cyprid settlement, and immunofluorescence analysis results for TGase.
From: New genes helped acorn barnacles adapt to a sessile lifestyle
a, The nine genes encode proteins that contain Tryp_SPc (trypsin) domains. The protein domains were predicted using a web server (http://pfam-legacy.xfam.org). b, The sequence alignment of Tryp_SPc domains of Homo sapiens thrombin and nine proteins. c, Ribbon drawings showing the structural similarity between Tryp_SPc domains of H. sapiens thrombin and that of nine proteins. Note that the comparison between the Tryp_SPc domain of one gene (AaSB) and H. sapiens thrombin has already been shown in Fig. 2e. d, Ribbon drawings showing the structural similarity between A. amphitrite transglutaminase (TGase, AlphaFold identifier: AF-A0A6A4WL68-F1) and different H. sapiens TGase. TGase1 (PDB: 1GGT) and TGase3 (PDB: 1L9M). e, Negative control of immunofluorescence analysis of TGase expression in the attachment disc of an A. amphitrite juvenile (representative images of n = 2 independent experiments).
