Extended Data Fig. 6: Alignment of bcs-6 transposon in A. amphitrite genome, and the location and expression of bcs-6 in P. pollicipes genome.
From: New genes helped acorn barnacles adapt to a sessile lifestyle
a, Sequence alignments of the U3 region in the 5′ LTR of 26 intact bcs-6 gene copies in the A. amphitrite genome. Cis-regulatory elements (CREs) were predicated by the web server AliBaba2.1. b, The locations of three intact bcs-6 gene copies and their expression levels in the P. pollicipes genome. The expression levels were calculated with published RNA-seq data (NCBI, no. PRJNA394196) from pooled larvae and adults of P. pollicipes and visualized by a tool in Integrative Genomics Viewer (IGV). c, The schematic diagrams of shedding cyprid shells of an acorn barnacle and a stalked barnacle, modified from video-based observations under the Creative Commons CC-BY-NC license40.
