Fig. 5: Model: a surface of EZH2 that can interact with either RNA or nucleosomes, rather than its RNA-binding activity per se, is required for maintaining the H3K27me3 mark at facultative heterochromatin. | Nature Genetics

Fig. 5: Model: a surface of EZH2 that can interact with either RNA or nucleosomes, rather than its RNA-binding activity per se, is required for maintaining the H3K27me3 mark at facultative heterochromatin.

From: Inseparable RNA binding and chromatin modification activities of a nucleosome-interacting surface in EZH2

Fig. 5

a, EZH2 mutants and their identified molecular properties, as determined herein. b, Model: PRC2 uses part of its RNA-binding surface to interact with chromatin during histone methylation (top left). At that point, the RNA-binding surface, not its RNA-binding activity, is being used for interactions with chromatin. When PRC2 is not methylating chromatin, EZH2 can use its RNA-binding surface to interact with RNA (bottom left). The molecular mechanism of how EZH2 mutants affect the canonical functions of PRC2 in H3K27me3 deposition (blue line) and transcriptional regulation (red line) in cells are illustrated in the top right: mt1 is indistinguishable from the wild-type EZH2, while mt2 phenocopy the catalytically defective EZH2 (dEZH2).

Back to article page