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Single-cell technology

Unveiling heterogeneity in rare cells by combining RNA-based sorting and scRNA-seq

Nature Genetics volume 57, pages 283–284 (2025)Cite this article

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A study describes the development of PERFF-seq, a method to ‘FISH’ out rare cells using RNA markers, helping to solve the challenge in single-cell biology of studying cells that make up less than 1% of a sample.

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Fig. 1: PERFF-seq enables isolation of rare populations for single-cell RNA sequencing.

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Authors and Affiliations

  1. Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, USA

    Madison Wahlsten & Sydney M. Shaffer

  2. Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Sydney M. Shaffer

Authors
  1. Madison Wahlsten
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  2. Sydney M. Shaffer
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Correspondence to Sydney M. Shaffer.

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The authors declare no competing interests.

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Wahlsten, M., Shaffer, S.M. Unveiling heterogeneity in rare cells by combining RNA-based sorting and scRNA-seq. Nat Genet 57, 283–284 (2025). https://doi.org/10.1038/s41588-024-02073-2

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