Extended Data Fig. 8: Comparative annotation and functional validation of the ZM113 private gene Ghir_D03G00738.

(a) IGV visualization of the TM-1 reference genome (version: CRI v1.0) region containing the unannotated Ghir_D03G00738 sequence. (b) IGV visualization of Ghir_D03G00738 annotation and transcriptome alignment in the ZM113 reference genome. (c) The VIGS results show that Ghir_D03G00738 regulates flowering time in upland cotton. Silenced plant phenotype images. (d) Silencing efficiency of Ghir_D03G00738 was detected by qRT-PCR. (e–g) Statistics of first fruiting node position, flowering time, and budding time.