Fig. 7: Sex DEG expression patterns in human kidneys. | Nature Genetics

Fig. 7: Sex DEG expression patterns in human kidneys.

From: Multi-omic and spatial analysis of mouse kidneys highlights sex-specific differences in gene regulation across the lifespan

Fig. 7

a, Diagram of overlapping male- and female-specific PT genes between mouse and human at various significance levels in human data (Methods; two-sided Wilcoxon rank-sum test Bonferroni method adjusted). b, Left, violin plot of SPP1 snRNA expression in PT. Right, violin and spatial plots of SPP1 expression from Visium ST. Yellow diamonds denote medians. c, Left, DOCK5 expression in PT of normal human kidney samples (two-sided Wilcoxon rank-sum test). Middle, DOCK5 expression in ccRCC tumor tissues compared to normal adjacent tissues. Boxplots show the median (centerline), first and third quartiles (hinges) and whiskers extending to values within 1.5× IQR from the hinges (two-sided Students’ t test). Right, Kaplan–Meier curves of the survival status of patients based on low/high DOCK5 expression, for males and females, separately (log-rank test). d–g, IF images and fluorescence intensity quantification of SOCS2 (d), SCD (e), CYP4B1 (f) and INMT (g) expression in female and male human kidney tissues (eight random areas, n = 3). LRP2 is used to label PT cells. Scale bar = 100 μm. Boxplots show the median (centerline), first and third quartiles (hinges) and whiskers extending to values within 1.5× IQR from the hinges (two-sided Welch’s t test). Representative images of the three biological replicates are in Supplementary Figs. 4–7. h, Nephron structure diagram. i, CODEX images of SOCS2, LRP2, AQP1, CALB1, UMOD and AQP2 in female and male human kidneys (n = 1 for each sex for demonstration purpose). Scale bar = 200 μm. j, CODEX images of SOCS2, LRP2 and AQP1 showing SOCS2 difference of female versus male in human kidneys. Scale bar = 100 μm. k, Mean SOCS2 intensity in LRP2+, UMOD+ and CALB1+ cells. Boxplots show the median (centerline), first and third quartiles (hinges) and whiskers extending to values within 1.5× IQR from the hinges (two-sided Welch’s t test). Quantification is performed on eight random regions from the multiplex image, repeated more than thrice with similar results. KIRC, kidney renal clear cell carcinoma; Expr, expression; Glom, glomeruli; IQR, interquartile range; TCGA, The Cancer Genome Atlas.

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