Extended Data Fig. 7: In vivo enhancer reporter assays test functional conservation of ortholog enhancer pairs. | Nature Genetics

Extended Data Fig. 7: In vivo enhancer reporter assays test functional conservation of ortholog enhancer pairs.

From: Conservation of regulatory elements with highly diverged sequences across large evolutionary distances

Extended Data Fig. 7

(a) Landing pad at the H11 Locus and enhancer targeting plasmid (b) Control Experiment using integration of a non-enhancer plasmid integrated at the H11 locus shows weak background signal in the otic vesicle, somites and the outflow tract at E10.5. (c) Genomic organization of the integrated enhancer-reporter construct at the H11 locus (d) Enhancer-reporter results for Nkx2-5 and Tbx20-IC heart enhancer pairs at E10.5 and 2 adult liver enhancer pairs from mouse and opossum tested in E11.5 embryos. (e) Summary of enhancer activity from all enhancer-reporter assays tested in this study. Scale bar: 1,000 µm (embryo) or 500 µm (heart).

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