Extended Data Fig. 1: Mean sequencing depth in the normal and chemotherapy exposed HSPC colonies used for mutation burden analysis, and patterns of mutation accumulation.
From: The long-term effects of chemotherapy on normal blood cells
a, Box plot representing the quartile distribution of mean sequencing depth in 90 colonies from normal (blue) and 189 colonies from chemotherapy exposed (red) individuals. The boxes indicate the median and interquartile range, the whiskers denote the minimum and maximum, with outlying values represented as points. b, Boxplot comparing the mean sequencing depth between Alkylating/ Platinum agent exposed and non-exposed colonies. The number of colonies in each agent group are shown at the top of the plot. The boxes indicate the median and interquartile range, the whiskers denote the minimum and maximum, with outlying values represented as points. c-f, HSC single base substitutions associated with chemotherapy (c,d) and age (e,f). CC, colorectal carcinoma; LC, lung cancer; NB, neuroblastoma; FL, follicular lymphoma; DLBL, diffuse large B cell lymphoma; MZL, marginal zone lymphoma; LL, lymphoplasmacytic lymphoma; M, multiple myeloma; HL, Hodgkin lymphoma; AML, acute myeloid leukaemia; 1, Platinum agents; 2,Alkylating agents; 3,Antimetabolites; 4,Topo I inhibitors; 5,Topo II inhibitors; 6,Vinca alkaloids; 7,Cytotoxic antibiotics.
