Extended Data Fig. 4: TACL-induced loop extrusion is RAD21 dependent.
From: Characterization of induced cohesin loop extrusion trajectories in living cells
(A) Western blot analysis of RAD21 in Cherry and TACL-ON RAD21-AID cells. DMSO serves as a control. RAD21 is degraded by 2 h of IAA treatment. GAPDH is used as loading control. (B) An example locus on chrX presenting the 4 C overlay and ChIP-seq tracks of FLAG, MAU2, NIPBL, SMC1, and PDS5A in TACL-ON RAD21-depleted cells or non-depleted cells (DMSO). The plot is centered at the 4 C viewpoint indicated as TetO in the lower part of the panel. (C) Relative enrichment of FLAG and NIPBL at the TetO platforms, calculated form ChIP-seq data. Values are normalized to TACL-OFF condition. Note the unchanged/increased binding of the factors in RAD21-depleted cells.
