Extended Data Fig. 9: HPLC chromatograms for the whole-cell reactions of the halogenase fusion enzymes (Fre-L3-SttH, Fre-L3-PyrH, and Fre-L3-RebH) after 24 h.
From: Production of Tyrian purple indigoid dye from tryptophan in Escherichia coli
Since the residual Trp was not present after the halogenation reaction in the consecutive two-cell reaction system, it was presumed that regular indigo byproduct would not be generated in the whole-cell reaction of the strain ΔtnaA TnaA+MaFMO. a and b: Authentic standards: Trp; phenol as an internal standard; 7-Cl-Trp (7-Cl); 5-Cl-Trp (5-Cl); and 6-Cl-Trp (6-Cl). Chlorination reaction of Fre-L3-PyrH (c), Fre-L3-SttH (d) and Fre-L3-RebH (e) using NaCl and Trp. F and G: Authentic standards: Trp; phenol as an internal standard; 7-Br-Trp (7-Br); 5-Br-Trp (5-Br); and 6-Br-Trp (6-Br). Bromination reaction of Fre-L3-PyrH (h), Fre-L3-SttH (i) and Fre-L3-RebH (j) using NaBr and Trp.
