Extended Data Fig. 8: Dimerization of YY1 promotes long-range DNA looping.
From: YY1 interacts with guanine quadruplexes to regulate DNA looping and gene expression
a, SDS-PAGE for monitoring the purified recombinant truncated forms of YY1 that is incapable of dimerization, but able to discriminate G4 structure from single-stranded DNA. b, Gel filtration chromatography revealed that YY1∆231-290 (calculated monomer MW: 38.4 kDa) exists as a monomer, and GST-YY1∆231-290 is present as a dimer (calculated monomer MW: 66.3 kDa). c-d, Fluorescence anisotropy for monitoring the binding of YY1∆231-290 and GST-YY1∆231-290 protein with G4 structure and the corresponding mutated sequence (M4) derived from the MYC promoter. e, Proximity ligation assay showing that YY1 and GST-YY1∆231-290, but not YY1∆231-290, is capable of promoting ligation between G4 DNA sequences.
