Extended Data Fig. 2: Live-cell ensemble FRET response to orthosteric ligands and a negative allosteric modulator.
From: Conformational rearrangement during activation of a metabotropic glutamate receptor
a, Representative donor and acceptor intensities and corresponding FRET signal from live-cell FRET titration experiments for DCG-IV, LY379268 and Glutamate +10 µM Ro64-5229 measured using HEK293T cells expressing 548UAA (top) and corresponding dose-response curves (bottom). Each titration curve is normalized to the 1 mM glutamate response. Data represent mean ± s.e.m. of N=20, 10 and 13 cells for LY379268, DCG-IV and glutamate +10 µM Ro64-5229, respectively, examined over 3 independent experiments. b, Donor and acceptor intensities and corresponding FRET signal in response to saturating concentrations of glutamate (1 mM), DCG-IV (100 µM) and LY379268 (20 µM) in HEK293T cells expressing 548UAA.
