Extended Data Fig. 7: Reduction of CycN by dithionite.
From: A cytochrome c is the natural electron acceptor for nicotine oxidoreductase
UV-VIS spectra were recorded as sodium dithionite was serially titrated into a solution of oxidized CycN until it became fully reduced. Arrows represent the directionality of change during the titration. Inset: zooming in on just a small section of this titration, an isosbestic point is visible at 542 nm marked with an arrow. This wavelength was used to monitor the changes in absorbance for NicA2’s FAD in the experiments in Fig. 4.
